Figure 3From: Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolutionSchematic of the screening protocol for improved methanol tolerance by directed evolution of PML. (A) Steps for colony lift assay and staining protocol. (B) Hydrolysis of 1-napthyl palmitate and detection of 1-naphthol by fast blue to assay colonies for residual activity after exposure to methanol and heat. (C) A representative filter following step 9 in (A). Black arrows point to potential positives identified on the filter.Back to article page