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Table 1 PCR analysis of the T 0 transgenic plants

From: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)

T0plant

Cry1Ab/1Ac 1

P1-P42

Hpt 3

pCCreloxPBt4

+

-

+

MD44

-

-

-

1

+

-

-

2

+

-

+

2B

+

-

+

4A

+

-

+

4B

+

-

+

6

+

-

+

8

+

-

+

10

+

+

+

11

+

+

+

12

+

-

+

13

+

-

+

19

+

-

+

20

+

+

-

21

+

+

+

24

+

-

+

33

+

+

+

37

+

+

+

38

+

+

+

39

+

-

+

40

+

-

+

  1. 1The primers for detecting the presence of the Cry1Ab/1Ac gene were Bt F1 and Bt R1; 2the primers for detecting the excision of the loxP fragment or the presence of P1-P4 fragment were P1 and P4 [34]; 3the primers for detecting the presence of the Hpt gene were Hpt F and Hpt910-1; 4binary construct used for transgenic J. curcas production [34].