T0plant |
Cry1Ab/1Ac
1
| P1-P42 |
Hpt
3
|
---|
pCCreloxPBt4 | + | - | + |
MD44 | - | - | - |
1 | + | - | - |
2 | + | - | + |
2B | + | - | + |
4A | + | - | + |
4B | + | - | + |
6 | + | - | + |
8 | + | - | + |
10 | + | + | + |
11 | + | + | + |
12 | + | - | + |
13 | + | - | + |
19 | + | - | + |
20 | + | + | - |
21 | + | + | + |
24 | + | - | + |
33 | + | + | + |
37 | + | + | + |
38 | + | + | + |
39 | + | - | + |
40 | + | - | + |
- 1The primers for detecting the presence of the Cry1Ab/1Ac gene were Bt F1 and Bt R1; 2the primers for detecting the excision of the loxP fragment or the presence of P1-P4 fragment were P1 and P4 [34]; 3the primers for detecting the presence of the Hpt gene were Hpt F and Hpt910-1; 4binary construct used for transgenic J. curcas production [34].