Figure 3

SDS-PAGE and Western blot characterization of Y. lipolytica transformants expressing the T. reesei , T. reesei-Talaromyces emersonii chimeric, and Chaetomium thermophilum . (A) Transformant expressing the T. reesei CBHI. (B) Transformant expressing the T. reesei-Talaromyces emersonii chimeric and C. thermophilum CBHIs. The supernatants were concentrated 300-fold and the loading amount was 20 μg proteins per well. Western blot analysis was conducted using anti-Tr CBHI antibody. The red dotted boxes indicate the band of expressed CBHI. Ct, Chaetomium thermophilum; Tr, Trichoderma reesei, Yl, Y. lipolytica.