Figure 4From: Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessingExpression profile and purification of chimeric CBHI protein in bioreactor fermentation of Y. lipolytica transformant. (A) Time course for the concentrated culture supernatant of Y. lipolytica[chimeric CBHI] transformant. The 25-fold concentrated supernatants (16 μL per well) were separated by SDS-PAGE, followed by Western blot analysis for the detection of chimeric CBHI. (B) SDS-PAGE and Western blot analyses for collected fractions during chimeric protein purification procedure. The chromatography fractions corresponding to gel lane numbers are: lane 1, the 20-fold concentrated culture supernatant (10 μL); lane 2, effluent from hydrophobic interaction chromatography (20 μL); lane 3, effluent from anion exchange column (20 μL); lanes 4 and 5, the minor impurity and peaks from size exclusion column (20 μL), respectively.Back to article page