Fig. 7
Working model for the genes/gene networks involved in fatty acid and TAG biosynthesis in Camelina sativa. The expression abundance (RPKMs in log2 scale) for the selected candidate genes are highlighted in different color scales in Camelina developing seeds at 10–15 days after flowering (Seed 14 DAF) and at 16–21 DAF (seed 21 DAF) as well as in leaf tissues. The full name for the metabolites shown in the pathways is pyruvate, acetyl-CoA, malonyl-CoA, malonyl-ACP acyl-carrier protein, Acetoacetyl-ACP, Acyl-ACP, sn-Glycerol 3-phosphate G3P, LPA lysophosphatidic acid, PA Phosphatidic acid, MAG monoacylglycerol, FAA free fatty acid, DAG 1,2-Diacylglycerol, TAG Triacylglycerol, PC phosphatidylcholine, LPC lysophosphatidylcholine, and glycerol. The enzymes shown here are pyruvate dehydrogenase E1-α (PDH-E1-α), PDH-E1-β pyrxuvate dehydrogenase E1-β, PDC pyruvate dehydrogenase complex, ACC1 Acetyl-CoA carboxylase, ACCase-α acetyl-CoA carboxylase, a-carboxyltransferase, EMP3147 acyl-carrier-protein S-malonyltransferase, KASI ketoacyl-ACP synthase I; KASII ketoacyl-ACP Synthase II; KASIII ketoacyl-ACP synthase III, WRl1 wrinkled 1, ENR1 enoyl-ACP reductase, ATFATA fatA acyl-ACP thioesterase, FATA acyl-ACP thioesterase A, FATB fatty-acyl-ACP thioesterase B, SAD stearoyl-ACP desaturase, LACS long chain Acyl-CoA synthase, NHO1 protein-similar to glycerol kinase, MGAT monoacylglycerol acyltransferase, AAPT1 Choline/ethanolaminephosphotransferase, GPAT9 glycerol-3-phosphate acyltransferase 9, LPAT2 lysophosphatidyl acyltransferase 2, SDP1 sugar-dependent protein, SDP1-L sugar-dependent 1-like protein, PAH1 phosphatidic acid phosphohydrolase 1, PAH2 phosphatidic acid phosphohydrolase 1, DGAT1 diacylglycerol O-acyltransferase 1, DGAT2 diacylglycerol O-acyltransferase 2, PDAT1 phospholipid:diacylglycerol acyltransferase 1, PDAT2 phospholipid:diacylglycerol acyltransferase 2, DGK diacylglycerol kinase, AGK acylglycerol kinase, MGLL acylglycerol lipase, PDCT phosphatidylcholine: diacylglycerol cholinephosphotransferase, AAPT aminoalcoholphosphotransferase, LPCAT1 lysophosphatidylcholine acyltransferase 1, LPCAT2 lysophosphatidylcholine acyltransferase 2, PLA2 phospholipase A2, Ole1 Oleosin1, Ole2 Oleosin 2, and Ole4 Oleosin 4. This model is modified from the model published by Wang and colleagues in 2012 [44], Dussert and colleagues in 2013 [45], and the glycerolipids metabolism pathway in KEGG database http://www.genome.jp/kegg/. The Gene/enzyme names are modified from the names available in the TAIR database (http://www.Arabidopsis.org) and the Camelina genome database (http://www.camelinadb.ca)