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Table 4 Lignin composition analysis of Norway spruce wood after 6 weeks of colonization by P. radiata

From: Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose

RT

Peak name

Spruce wood (%)

After 6 weeks of fungal growth (%)

Change (%)

Mean

Mean

7.126

Phenol (hydroxybenzene)

0.29 ± 0.08

0.50 ± 0.09

+72

8.401

2-methylphenol

0.11 ± 0.03

0.16 ± 0.03

+45

8.753

4-methylphenol, (4-methylguaiacol)

0.25 ± 0.06

0.33 ± 0.05

+32

9.01

2-methoxyphenol, (Guaiacol)

3.33 ± 0.24

4.69 ± 0.30

+41

10.437

2-methoxy-3-methylphenol

0.76 ± 1.22

1.24 ± 0.99

+63

10.64

2-methoxy-4-methylphenol

3.19 ± 0.47

2.44 ± 0.47

−24

11.89

2-methoxy-4-ethylphenol, (ethylguaiacol)

0.83 ± 0.10

0.73 ± 0.08

−18

12.428

4-vinylguaiacol

3.17 ± 0.29

3.22 ± 0.39

+2

12.994

Eugenol, 4-allylguaiacol

0.57 ± 0.05

0.45 ± 0.02

−21

13.129

2-methoxy-4-propylphenol, (4-propylguaiacol)

0.31 ± 0.03

0.26 ± 0.04

−16

13.626

Vanillin

1.27 ± 0.56

1.45 ± 0.71

+14

13.692

2-methoxy-4-(1-propenyl)phenol, (isoeugenol, cis)

0.27 ± 0.02

0.23 ± 0.02

−15

14.274

2-methoxy-4-(1-propenyl)phenol, (isoeugenol, trans)

1.78 ± 0.18

1.47 ± 0.25

−17

14.355

1-(4-hydroxy-3-methoxyphenyl)ethanone, (guaiacylacetone)

1.06 ± 0.65

0.86 ± 0.65

−19

14.733

1-(4-hydroxy-3-methoxyphenyl)ethanone (acetovanillone)

0.98 ± 0.46

0.54 ± 0.47

−45

15.201

Phenol, 2-methoxy-4-propan-1-ol, (dihydroconiferyl alcohol)

0.81 ± 0.54

0.35 ± 0.27

−57

15.749

Coniferyl alcohol, cis

0.47 ± 0.25

0.24 ± 0.17

−49

15.867

1-(4-hydroxy-3-methoxyphenyl)propanone, (propiovanillone)

0.58 ± 0.27

0.35 ± 0.30

−40

16.657

Coniferyl alcohol, trans

0.60 ± 0.46

0.35 ± 0.46

−42

17.673

Coniferyl aldehyde

0.51 ± 0.35

0.52 ± 0.59

+2

Pyrolysis lignin (area aromatics/total)

21.3 ± 0.7

20.4 ± 2.0

−4

Gravimetric (Klason) lignin

25.8 ± 0.33

26.1 ± 0.51

+1

Acid soluble lignin

0.30 ± 0.04

0.68 ± 0.23

+127

Pch-C1,2/Pch-C3 ratioa

0.03

0.08

+167

  1. Relative peak areas (%) of lignin-derived pyrolysis product compounds identified with pyrolysis–GC/MS. Gravimetric and acid soluble lignin contents are also shown. The values are calculated from two biological replicates where 3–4 technical replicates were taken for pyrolysis samples, and from three biological replicates each of which with two technical replicate samples for Klason lignin determination
  2. aRatio of phenylmethane and phenylethane to phenylpropane type compounds