Fig. 3
From: Identification of two integration sites in favor of transgene expression in Trichoderma reesei
SDS-PAGE and Western blot analysis of the secreted proteins and the intracellular RFP. a SDS-PAGE analysis of the supernatant of 11 representative sorted recombinant strains from 120Â h cultures containing MM media with 1% Avicel (w/v) as the carbon source from 24 well plates. b Western blot analysis of the intracellular RFP expression. Actin protein was used as a reference to normalize the biomass. Protein samples were extracted from mycelia in the same culture as that in a. The raw data was shown in Additional file 2: Figure S2