Fig. 5

Analysis of HmgR Protein Abundance vs. Isopentenol Titer. All strains were prepared for isopentenol production in CGXII medium at the % (w/v) d-glucose concentration indicated below the graph. a Proteomic analysis of HmgR protein abundance: Absolute abundance for HmgR at the timepoints indicated are plotted as a function of cultivation condition. b Correlation analysis between isopentenol titer and HmgR protein abundance. Correlation was determined using a linear regression for the Pearson correlation coefficient (PCC) for the two variables. c Role of increased HMGR expression on isopentenol titer. All strains were cultivated for isopentenol production in CGXII medium amended with 4% d-glucose concentration in 24-well deep well plates. White bars: C. glutamicum ∆idsA harboring the plasmid-borne isopentenol production pathway. Grey hatched bars: ∆idsA with a chromosomally integrated isopentenol production pathway at the idsA locus. Green bars: ∆idsA with the same chromosomally encoded isopentenol production pathway as well as a plasmid-borne HMGR overexpression cassette. For all experiments, data was generated from three independent biological replicates for each condition, and error bars indicate standard error