Fig. 4From: Mutation of 4-coumarate: coenzyme A ligase 1 gene affects lignin biosynthesis and increases the cell wall digestibility in maize brown midrib5 mutantsEffect of Zm4CL1 mutations on 4CL enzyme activities. Crude enzyme extracts were prepared from the midribs of B73, bm5-504I, and bm5-504J. The 4CL enzyme activities of the above crude enzyme extracts against p-coumarate, caffeate, and ferulate were determined. Midribs of the fourth leaves were collected from 60-day-old B73 wild-type and bm5 mutantsBack to article page