Fig. 4

Activity of mgLPMO10 and ScLPMO10C at various temperatures. a–e show progress curves for copper-saturated mgLPMO10 (solid curves) and ScLPMO10C (dashed curves) at 40, 50, 60, 70 and 80 °C, respectively. The reactions were carried out with 1 µM LPMO and 10 g/L Avicel in 50 mM sodium phosphate buffer pH 6.0. The reactions were pre-incubated for 10 min at various temperatures before 1 mM ascorbic acid was added to start the LPMO reaction. The reactions were carried out in Eppendorf Thermomixers at 800 rpm and samples were taken and filtered at 10, 20, 30, 60 and 120 min. The solubilized products were degraded to oxidized dimers and trimers by incubation with 0.5 µM mgCel6A prior to product quantification. The reaction with ScLPMO10C at 80 °C (e) did not yield any oxidized products. The error bars show ± SD (n = 3)