Development of a hybrid bio-purification process of lactic acid solutions employing an engineered E. coli strain in a membrane bioreactor

Table 1 Experimental design of semi-continuous MBR experiments

		MBR 1	MBR 2	MBR 3	MBR 4	MBR 5 & 6
Overall process conditions	Nutrients in batch	25% Μ9 + TE	25% M9 + TE	25% M9 + TE	50% M9 + TE	50% M9 + TE
Overall process conditions	Nutrients in MBR	25% Μ9 + TE	25% Μ9 + TE	50% M9 + TE	50% M9 + TE	50% M9 + TE
Batch mode	Fermentation time	24 h	30 h	30 h	24 h	34 h
Semi-continuous MBR mode
Cycle 1	Permeate/Feed volume	300 mL	300 mL	300 mL	230 mL	230 mL
Cycle 1	Fermentation time	7 h	16 h	16 h	3 h	3 h
Cycle 2	Permeate/Feed volume	300 mL	200 mL	200 mL	230 mL	230 mL
Cycle 2	Fermentation time	7 h	8 h	8 h	3 h	3 h
Cycle 3	Permeate/Feed volume	600 mL	300 mL	300 mL	230 mL	230 mL
Cycle 3	Fermentation time	14 h	16 h	16 h	3 h	3 h
Cycle 4	Permeate/Feed volume	300 mL	200 mL	200 mL	230 mL	230 mL
Cycle 4	Fermentation time	7 h	16 h	16 h	3 h	3 h
Cycle 5	Permeate/Feed volume	-	300 mL	300 mL	-	-
Cycle 5	Fermentation time	-	16 h	16 h	-	-
Cycle 6	Permeate/Feed volume	-	200 mL	-	-	-
Cycle 6	Fermentation time	-	16 h	-	-	-

ISSN: 2731-3654