Table 4 Strains and plasmids used in this study

Strains

Escherichia coli

DH5α

Cloning host: F-λ-endA1 glnX44(AS) thiE1 recA1 relA1 spoT1 gyrA96(Nal^R) rfbC1 deoR nupG Φ80(lacZΔM15) Δ(argF-lac)U169 hsdR17(r_K^–m_K⁺)

[35]

Pseudomonas putida

DOT-T1E

Prototroph, Cm^R, Rif^R

[8]

DOT-T1EΔgcd

DOT-T1E mutant generated by insertional inactivation of DOT-T1E_2882, Km^R

[34]

DOT-T1E (xylABE)

Wild type harboring plasmid pSEVA633_xylABE, Gm^R

[34]

DOT-T1EΔgcd (xylABE)

DOT-T1E Δgcd mutant harboring plasmid pSEVA633_xylABE, Km^R, Gm^R

[34]

CM12-5

DOT-T1E mutant that produces L-phenylalanine, Rif^R

[24]

CM12-5Δgcd

CM12-5 mutant generated by insertional inactivation of DOT-T1E_2882, Km^R

[34]

CM12-5 (xylABE)

CM12-5 harboring plasmid pSEVA633_xylABE, Gm^R

[34]

CM12-5Δgcd (xylABE)

CM12-5 Δgcd harboring plasmid pSEVA633_xylABE, Gm^R

[34]

PG1E

CM12-5 derivative that grows in the presence of 10 mg mL⁻¹ of FPA, Rif^R

This study

PG2E

CM12-5 (pPHE1) derivative that grows in the presence of 10 mg mL⁻¹ of FPA, Rif^R Km^R

This study

Plasmids

pSEVA238

Expression vector: oriV(pBBR1),

xylS/ Pm, Km^R

[67]

pPHE1

pSEVA238 derivative carrying pheA^fbr gene from P. putida DOT-T1E

[24]

pSEVA633

Expression vector: oriV(pBBR1), lacZα-pUC18, Gm^R

[67]

pSEVA633_xylABE

pSEVA633 with xylABE genes expressed from the EM7 promoter, Gm^R

[34]

pSEVA438

Expression vector: oriV(pBBR1), xylS/Pm, Sm/Sp^R

[67]

pPE-1

pSEVA438 derivative carrying PP_0968 from P. putida KT2440, kdc from Rhodospirillum rubrum,

and T1E_5478 from P. putida DOT-T1E, Sm^R/Sp^R

[24]

pSEVA433

Expression vector: oriV(pBBR1), lacZα-pUC18, Sm^R/Sp^R

[67]

pPE-2

pSEVA433 derivative carrying PP_0968 from P. putida KT2440, kdc from Rhodospirillum rubrum,

and T1E_5478 from P. putida DOT-T1E, Sm^R/Sp^R

This study